Ventral and lateral regions of the zebrafish gastrula, including the neural crest progenitors, are established by abmp2b/swirlPathway of genes
VH Nguyen, B Schmid, J Trout, SA Connors… - Developmental …, 1998 - Elsevier
VH Nguyen, B Schmid, J Trout, SA Connors, M Ekker, MC Mullins
Developmental biology, 1998•ElsevierA bone morphogenetic protein (BMP) signaling pathway is implicated in dorsoventral
patterning inXenopus. Here we show that three genes in the zebrafish, swirl, snailhouse,
andsomitabun, function as critical components within a BMP pathway to pattern ventral
regions of the embryo. The dorsalized mutant phenotypes of these genes can be rescued by
overexpression ofbmp4, bmp2b, an activated BMP type I receptor, and the downstream
functioningSmad1gene. Consistent with a function as a BMP ligand, swirlfunctions cell …
patterning inXenopus. Here we show that three genes in the zebrafish, swirl, snailhouse,
andsomitabun, function as critical components within a BMP pathway to pattern ventral
regions of the embryo. The dorsalized mutant phenotypes of these genes can be rescued by
overexpression ofbmp4, bmp2b, an activated BMP type I receptor, and the downstream
functioningSmad1gene. Consistent with a function as a BMP ligand, swirlfunctions cell …
A bone morphogenetic protein (BMP) signaling pathway is implicated in dorsoventral patterning inXenopus.Here we show that three genes in the zebrafish,swirl, snailhouse,andsomitabun,function as critical components within a BMP pathway to pattern ventral regions of the embryo. The dorsalized mutant phenotypes of these genes can be rescued by overexpression ofbmp4, bmp2b,an activated BMP type I receptor, and the downstream functioningSmad1gene. Consistent with a function as a BMP ligand,swirlfunctions cell nonautonomously to specify ventral cell fates. Chromosomal mapping ofswirland cDNA sequence analysis demonstrate thatswirlis a mutation in the zebrafishbmp2bgene. Interestingly, our analysis suggests that the previously described nonneural/neural ectodermal interaction specifying the neural crest occurs through a patterning function ofswirl/bmp2bduring gastrulation. We observe a loss in neural crest progenitors inswirl/bmp2bmutant embryos, whilesomitabunmutants display an opposite, dramatic expansion of the prospective neural crest. Examination of dorsally and ventrally restricted markers during gastrulation reveals a successive reduction and reciprocal expansion in nonneural and neural ectoderm, respectively, insnailhouse, somitabun,andswirlmutant embryos, withswirl/bmp2bmutants exhibiting almost no nonneural ectoderm. Based on the alterations in tissue-specific gene expression, we propose a model wherebyswirl/bmp2bacts as a morphogen to specify different cell types along the dorsoventral axis.
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