[11] Purification of mitochondrial DNA from human cell cultures and placenta
C Ausenda, A Chomyn - Methods in enzymology, 1996 - Elsevier
Publisher Summary This chapter explains the purification of mitochondrial DNA from human
cell cultures and placenta. The need for mitochondrial DNA (mtDNA)-specific probes for the
detection and analysis of mitochondrial RNA and mtDNA is usually satisfied by available
cloned fragments of human mtDNA or by polymerase chain reaction (PCR)-amplified
segments of mtDNA. On the other hand, there are instances, in which it is desirable to use
the total mitochondrial genome as a hybridization probe. Thus, purified human mtDNA is …
cell cultures and placenta. The need for mitochondrial DNA (mtDNA)-specific probes for the
detection and analysis of mitochondrial RNA and mtDNA is usually satisfied by available
cloned fragments of human mtDNA or by polymerase chain reaction (PCR)-amplified
segments of mtDNA. On the other hand, there are instances, in which it is desirable to use
the total mitochondrial genome as a hybridization probe. Thus, purified human mtDNA is …