[HTML][HTML] Sphingomyelin synthase, a potential regulator of intracellular levels of ceramide and diacylglycerol during SV40 transformation: does sphingomyelin synthase …
C Luberto, YA Hannun - Journal of Biological Chemistry, 1998 - ASBMB
Sphingomyelin synthase (SMS), an enzyme involved in sphingomyelin (SM) and ceramide
metabolism, can potentially regulate, in opposite directions, the levels of ceramide and
diacylglycerol. In this study SMS activity was investigated in normal and SV40-transformed
human lung fibroblasts (WI38). The addition of [3 H] C 2-ceramide to cells resulted in a time-
dependent formation of [3 H] C 2-SM. At 24 h after treatment, normal WI38 cells cleared 17%
of [3 H] C 2-ceramide producing [3 H] C 2-SM, which accounted for 13% of total radioactivity …
metabolism, can potentially regulate, in opposite directions, the levels of ceramide and
diacylglycerol. In this study SMS activity was investigated in normal and SV40-transformed
human lung fibroblasts (WI38). The addition of [3 H] C 2-ceramide to cells resulted in a time-
dependent formation of [3 H] C 2-SM. At 24 h after treatment, normal WI38 cells cleared 17%
of [3 H] C 2-ceramide producing [3 H] C 2-SM, which accounted for 13% of total radioactivity …