[HTML][HTML] Efficient trafficking of ceramide from the endoplasmic reticulum to the Golgi apparatus requires a VAMP-associated protein-interacting FFAT motif of CERT
M Kawano, K Kumagai, M Nishijima… - Journal of Biological …, 2006 - ASBMB
Ceramide is synthesized at the endoplasmic reticulum (ER) and transported to the Golgi
apparatus by CERT for its conversion to sphingomyelin in mammalian cells. CERT has a
pleck-strin homology (PH) domain for Golgi targeting and a START domain catalyzing the
intermembrane transfer of ceramide. The region between the two domains contains a short
peptide motif designated FFAT, which is supposed to interact with the ER-resident proteins
VAP-A and VAP-B. Both VAPs were actually co-immunoprecipitated with CERT, and the …
apparatus by CERT for its conversion to sphingomyelin in mammalian cells. CERT has a
pleck-strin homology (PH) domain for Golgi targeting and a START domain catalyzing the
intermembrane transfer of ceramide. The region between the two domains contains a short
peptide motif designated FFAT, which is supposed to interact with the ER-resident proteins
VAP-A and VAP-B. Both VAPs were actually co-immunoprecipitated with CERT, and the …