[HTML][HTML] Analysis and regulation of vasodilator-stimulated phosphoprotein serine 239 phosphorylation in vitro and in intact cells using a phosphospecific monoclonal …
A Smolenski, C Bachmann, K Reinhard… - Journal of Biological …, 1998 - ASBMB
The development and functional analysis of a monoclonal antibody (16C2) are reported; the
antibody recognizes vasodilator-stimulated phosphoprotein (VASP; an established substrate
of both cAMP-and cGMP-dependent protein kinase) only when serine 239 is
phosphorylated. VASP serine 239 represents one of the best characterized cGMP-
dependent protein kinase phosphorylation sites in vitro and in intact cells. Experiments with
purified, recombinant human VASP and various VASP constructs with mutated …
antibody recognizes vasodilator-stimulated phosphoprotein (VASP; an established substrate
of both cAMP-and cGMP-dependent protein kinase) only when serine 239 is
phosphorylated. VASP serine 239 represents one of the best characterized cGMP-
dependent protein kinase phosphorylation sites in vitro and in intact cells. Experiments with
purified, recombinant human VASP and various VASP constructs with mutated …