Although computer-assisted sperm analysis (CASA) outperforms manual techniques, many
investigators rely on non-automated analysis due to the high cost of commercial options. In
this study, we have written and validated a free CASA software primarily for analysis of fish
sperm. This software is a plugin for the free National Institutes of Health software ImageJ and
is available with documentation at. That it is open source makes possible external validation,
should improve quality control and enhance the comparative value of data obtained among …