[HTML][HTML] IP3 receptors and Ca2+ signals in adult skeletal muscle satellite cells in situ
Biological research, 2004•SciELO Chile
In this short article we review muscle satellite cell characteristics and our studies in adult
rodent muscle satellite cells in situ. Using confocal laser scanning microscopy and
immunocytochemistry, a high level of IP 3 receptor (IP 3 R) immunostaining was detected in
satellite cells. These cells were identified by their peripheral position, their size, the shape of
their nucleus, the paucity of the apparent cytoplasm, and the immunostaining with specific
molecular markers such as a-actinin, the neural cell adhesion molecule (N-CAM) and …
rodent muscle satellite cells in situ. Using confocal laser scanning microscopy and
immunocytochemistry, a high level of IP 3 receptor (IP 3 R) immunostaining was detected in
satellite cells. These cells were identified by their peripheral position, their size, the shape of
their nucleus, the paucity of the apparent cytoplasm, and the immunostaining with specific
molecular markers such as a-actinin, the neural cell adhesion molecule (N-CAM) and …
Abstract
In this short article we review muscle satellite cell characteristics and our studies in adult rodent muscle satellite cells in situ. Using confocal laser scanning microscopy and immunocytochemistry, a high level of IP 3 receptor (IP 3 R) immunostaining was detected in satellite cells. These cells were identified by their peripheral position, their size, the shape of their nucleus, the paucity of the apparent cytoplasm, and the immunostaining with specific molecular markers such as a-actinin, the neural cell adhesion molecule (N-CAM) and desmin. High extracellular K+(60 mM) induced long-lasting Ca 2+ signals in satellite cells in situ. We suggest that electrical activity stimulates IP 3-associated Ca 2+ signals that could act in concert with signaling pathways triggered by growth factors and/or hormones.
SciELO Chile
